Collection Manuals: Urine for Cytology


Description: Urinary tract cytology is the microscopic evaluation of the solid components of urine, primarily to assess the patient’s status in regards to urothelial carcinoma and renal disease. Sample collection is from voided urine, bladder catheterization or wash and by renal barbotage.

MATERIALS
CytoLyt™ Solution (120 ml pre-filled cup. CAUTION: POISON, FLAMMABLE). Requisition form, biohazard transport bag.

PREPARATION
1. Complete a requisition form.
White copy to the lab. Lack of data may result in delay or specimen rejection. Include:
• Clinician name, location and date of service.
• Patient demographics: Name, address, phone number, date of birth and social security number.
• Insurance information: copy of insurance card(s) or face sheet.
• Method of specimen collection, for example; voided urine, catheter or wash, renal barbotage.
• Relevant patient history i.e., hematuria, fever, history of UTI, history of bladder cancer, history of renal disease,
  CT scan findings and suspected diagnosis.
• Related treatments including chemotherapy, radiation and surgery.

2. Label the cup with the patient’s first and last name.

COLLECTING THE SPECIMEN
3. Voided urine specimens, reduce contamination of the sample.
• The area around the urethral opening is a source of cellular and organism contaminants.
• Provide the patient a moist towelette with instructions to wipe the area around the urethral opening.

4. Collect 20 cc to 80 cc of urine.
• For voided urine instruct the patient to collect a mid-stream specimen directly into the pre-filled CytoLyt jar.
• Do not collect the first urine of the day. The second urine is preferred.

PACKAGING FOR TRANSPORT
5. Verify that the lid is properly seated and tight.

6. Submit in a biohazard transport bag.
• Submit the cup in the zip-loc portion of the transport bag and the requisition form in the outer sleeve.

7. Refrigeration is not required.
Specimens for microbiology or culture & sensitivity should be collected separately of cytology specimens and should NOT be submitted in cytology fixative. Refer to your clinical laboratory for collection procedures.