Collection Manual: Bronchial wash for cytology


Description: Bronchial wash specimens are collected at bronchoscopy and submitted for cytologic evaluation of bronchial fluids, often as an adjunct to bronchial brushing and biopsy. Whereas the brush and biopsy specimens are directed at a specific target, the wash can provide a broader sample of the bronchial pathology, including organisms. The bronchial wash should be collected after the brush specimen.

MATERIALS
CytoLyt™ Solution (pre-filled 120ml jar. CAUTION: POISON, FLAMMABLE). Requisition form, biohazard transport bag.

PROCEDURE
1. Complete a requisition form.
White copy to the lab. Lack of data may result in delay or specimen rejection.
Include:
• Clinician name, location and date of service.
• Patient demographics: Name, address, phone number, date of birth and social security number.
• Insurance information: copy of insurance card(s) or face sheet.
• Site of specimen, for example RUL, RML, LLL etc.
• Relevant patient history i.e., shortness of breath, hemoptysis, weight loss, previously diagnosed pulmonary disease,
  x-ray findings and suspected diagnosis.
• Related treatments including antibiotics, radiation, chemotherapy and surgery.
• Request for silver stain if mycosis or pneumocystis is suspected.

2. Label the jar with the patient’s first and last name.

3. Collect the wash into the specimen jar.
• Put the specimen directly into the pre-filled CytoLyt collection jar for optimum specimen integrity.

4. Cap the sample jar, verifying that the lid is properly seated and tight.

5. Submit in a biohazard transport bag.
• Submit the jar in the zip-loc portion of the transport bag and the requisition form in the outer sleeve.

6. Refrigeration is not required.
Specimens for microbiology or culture should be collected separately of cytology specimens and should NOT be submitted in cytology fixative. Refer to your clinical laboratory for collection procedures.